Extinction of alpha-fetoprotein gene expression in somatic cell hybrids involves cis-acting DNA elements.
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چکیده
منابع مشابه
Extinction of alpha 1-antitrypsin gene expression in somatic cell hybrids: evidence for multiple controls.
Expression of the liver-specific alpha 1-antitrypsin (alpha 1AT) gene is extinguished in hepatoma/fibroblast hybrids. To define the mechanism of extinction, we identified DNA sequences involved in this process by transiently transfecting mutant alpha 1AT promoters into parental and hybrid cells. The wild-type alpha 1AT promoter (-554 to +44 bp) was highly expressed in rat hepatoma cells, but ac...
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Extinction in somatic cell hybrids is a multifactorial process that leads to loss of cell-type-specific gene expression. The underlying mechanisms are thought to mirror, at least in part, the repertoire of regulatory mechanisms controlling mammalian cell differentiation.
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Expression of the liver-specific a,-antitrypsin (a,AT) gene is extinguished in hepatoma/fibroblast hybrids. To define the mechanism of extinction, we identified DNA sequences involved in this process by transiently transfecting mutant a^AT promoters into parental and hybrid cells. The wild-type WjAT promoter ( -554 to +44 bp) was highly expressed in rat hepatoma cells, but activity was 100-fold...
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1 1.1 Cis-acting elements in somatic hypermutation 3 1.2 Transacting elements in somatic hypermutation 4 1.2.1 Activation-induced cytidine deaminase (AID) 4 1.2.2 Mismatch repair in somatic hypermutation 9 1.3 Mutation rates measurements 13 1.4. Specific Aims 15 1.4.1. Specific aim 1: Develop a retroviral based point mutation indicator system 15 1.4.2. Specific aim 2: Characterize the IgH major...
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ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 1987
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.7.7.2606